Controlled Reverse Translocation of DNA Through a Solid - State

Submitted for the MAR10 Meeting of The American Physical Society Controlled Reverse Translocation of DNA Through a Solid-State Nanopore1 PAUL WEINGER, VENKAT BALAGURUSAMY, SUNGCHEOL KIM, XINSHENG SEAN LING, Brown University — Reverse DNA translocation [1] is a process in which a DNA is pulled out of a nanopore against the forward electric force. In this process, the effective diffusion constant of the DNA is significantly reduced, thereby suppressing thermal smearing effect due to diffusion in the positional measurements of DNA sequences, e.g. in the hybridization-assisted nanopore sequencing platform. We describe a new experimental setup which will provide dramatic improvement upon the previous experiment [1]. [1] Hongbo Peng and X.S. Ling, “Reverse DNA translocation through a solid-state nanopore by magnetic tweezers,” Nanotechnology, 20, 185101(2009). 1This work was supported by the NHGRI. Xinsheng Ling Brown University Date submitted: 24 Nov 2009 Electronic form version 1.4